Mass Spectrometry of Glycoproteins Methods and Protocols /

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 Glycosylation is the most abundant post-translational modification of proteins.  Estimates vary widely, but a common assessment is that upwards of 50% of eukaryotic proteins are modified by some type of glycan. In Mass Spectrometry of Glycoproteins: Methods and Protocols, expert researchers in the...

Ausführliche Beschreibung

Gespeichert in:
Hauptverfasser:
Kohler, Jennifer J. (HerausgeberIn), Patrie, Steven M. (HerausgeberIn)
Körperschaft:
SpringerLink (Online service)
Format:
Elektronisch E-Book
Sprache:
Englisch
Veröffentlicht:
Totowa, NJ : Humana Press : Imprint: Humana, 2013.
Ausgabe:
1st ed. 2013.
Zusammenfassung:
 Glycosylation is the most abundant post-translational modification of proteins.  Estimates vary widely, but a common assessment is that upwards of 50% of eukaryotic proteins are modified by some type of glycan. In Mass Spectrometry of Glycoproteins: Methods and Protocols, expert researchers in the field detail many of the methods that are now commonly used for glycoproteomics.  These methods and techniques include robust sample preparation techniques; advanced chromatographic strategies for improving dynamic range; state-of-the-art mass spectrometry instrumentation and associated ionization and fragmentation methods; and informatics tools used for identifying glycoproteins and characterizing the associated glycans. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.   Authoritative and practical, Mass Spectrometry of Glycoproteins: Methods and Protocol  is  an essential resource for those who work at the interface of glycobiology and mass spectrometry.
Umfang:
1 Online-Ressource (XI, 339 Seiten) 90 Illustrationen, 12 Illustrationen in Farbe
text file PDF
ISBN:
9781627031462
ISSN:
1940-6029 ;
DOI:
10.1007/978-1-62703-146-2
Schlagworte:
Proteins .
Mass spectroscopy.
Protein Biochemistry.
Mass Spectrometry.
Bezugswerke:
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