Barley Methods and Protocols /

This detailed volume explores barley as both a crop and a model, with practical techniques such as crossing barley, a range of tissue culture methods, the preparation of barley tissues for different forms of microscopy, and the assessment of sensitivity to abiotic stresses. Efficient protocols are p...

Ausführliche Beschreibung

Gespeichert in:
1. Verfasser:
Harwood, Wendy A. (HerausgeberIn)
Körperschaft:
SpringerLink (Online service)
Format:
Elektronisch E-Book
Sprache:
Englisch
Veröffentlicht:
New York, NY : Springer New York : Imprint: Humana, 2019.
Ausgabe:
1st ed. 2019.
Zusammenfassung:
This detailed volume explores barley as both a crop and a model, with practical techniques such as crossing barley, a range of tissue culture methods, the preparation of barley tissues for different forms of microscopy, and the assessment of sensitivity to abiotic stresses. Efficient protocols are provided for transformation, TILLING, virus-induced gene silencing and genome editing. There is also particular emphasis on a range of protocols for genotyping and for the analysis of gene expression. Written for the highly successful Methods in Molecular Biology series, chapters include introductions on their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and easy-to-use, Barley: Methods and Protocols serves as a valuable reference volume for cereal researchers and breeders by providing detailed protocols covering important traditional skills such as crossing and tissue culture through to the latest technologies for genotyping, expression analysis, and genome editing.
Umfang:
1 Online-Ressource (XI, 313 Seiten) 113 Illustrationen, 95 Illustrationen in Farbe
text file PDF
ISBN:
9781493989447
ISSN:
1940-6029 ;
DOI:
10.1007/978-1-4939-8944-7
Schlagworte:
Plant biotechnology.
Agriculture.
Plant Biotechnology.
Bezugswerke:
Links: