Directed Evolution Methods and Protocols /

This volume explores the latest techniques used by researchers to study directed evolution (DE) at each stage of the Design-Build-Test-Learn cycle. Chapters in this book cover topics such as designing overlap extension PCR primers for protein mutagenesis; antha-guided automation of Darwin assembly f...

Ausführliche Beschreibung

Gespeichert in:
Hauptverfasser:
Currin, Andrew., Swainston, Neil.
Körperschaft:
SpringerLink (Online service)
Weitere Verfasser:
Currin, Andrew (HerausgeberIn), Swainston, Neil (HerausgeberIn)
Format:
Elektronisch E-Book
Sprache:
Englisch
Veröffentlicht:
New York, NY : Springer US : Imprint: Humana, 2022.
Ausgabe:
1st ed. 2022.
Zusammenfassung:
This volume explores the latest techniques used by researchers to study directed evolution (DE) at each stage of the Design-Build-Test-Learn cycle. Chapters in this book cover topics such as designing overlap extension PCR primers for protein mutagenesis; antha-guided automation of Darwin assembly for the construction of bespoke gene libraries; rapid cloning of random mutagenesis libraries using PTO-Quickstep; and DE of glycosyltransferases by a single-cell screening method. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Directed Evolution: Methods and Protocols is a valuable resource for scientists and researchers who are interested in learning more about this field and incorporating these studies into new experimental workflows.
Umfang:
1 Online-Ressource (XI, 278 Seiten) 63 Illustrationen, 58 Illustrationen in Farbe
text file PDF
ISBN:
9781071621523
ISSN:
1940-6029 ;
DOI:
10.1007/978-1-0716-2152-3
Schlagworte:
Biotechnology.
Medical genetics.
Medical Genetics.
Bezugswerke:
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