Heterologous Expression of Membrane Proteins Methods and Protocols /

Weitere Versionen (3)

This detailed volume explores protocols for the production of membrane proteins in a panel of heterologous organisms for structural studies. Beginning with techniques using E. coli as a host for the overproduction and purification of membrane proteins, the book continues with chapters covering mamma...

Ausführliche Beschreibung

Gespeichert in:
1. Verfasser:
Mus-Veteau, Isabelle.
Körperschaft:
SpringerLink (Online service)
Weitere Verfasser:
Mus-Veteau, Isabelle (HerausgeberIn)
Format:
Elektronisch E-Book
Sprache:
Englisch
Veröffentlicht:
New York, NY : Springer US : Imprint: Humana, 2022.
Ausgabe:
3rd ed. 2022.
Zusammenfassung:
This detailed volume explores protocols for the production of membrane proteins in a panel of heterologous organisms for structural studies. Beginning with techniques using E. coli as a host for the overproduction and purification of membrane proteins, the book continues with chapters covering mammalian membrane protein production in yeast, insect cells, mammalian cells, as well as using virus like particles and acellular systems. Additionally, new detergents and alternatives to detergents allowing membrane protein purification for structural analyses are described. The book closes with a chapter exploring the use of microscale thermophoresis (MST) to evaluate the binding activity of heterologously expressed proteins directly in crude membrane extracts. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Heterologous Expression of Membrane Proteins: Methods and Protocols, Third Edition serves as an ideal guide for scientists aiming to produce and purify functional recombinant membrane proteins for structural studies.
Umfang:
1 Online-Ressource (XVII, 467 Seiten) 94 Illustrationen, 78 Illustrationen in Farbe
text file PDF
ISBN:
9781071623688
ISSN:
1940-6029 ;
DOI:
10.1007/978-1-0716-2368-8
Schlagworte:
Membranes (Biology).
Proteins.
Biological Membranes.
Bezugswerke:
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