Heterologous Gene Expression in E.coli Methods and Protocols /

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This detailed volume provides a toolbox for designing constructs, tackling expression and solubility issues, handling membrane proteins and protein complexes, and exploring innovative engineering of E. coli. The topics are largely grouped under four parts: high-throughput cloning, expression screeni...

Ausführliche Beschreibung

Gespeichert in:
1. Verfasser:
Burgess-Brown, Nicola A. (HerausgeberIn)
Körperschaft:
SpringerLink (Online service)
Format:
Elektronisch E-Book
Sprache:
Englisch
Veröffentlicht:
New York, NY : Springer New York : Imprint: Humana, 2017.
Ausgabe:
1st ed. 2017.
Zusammenfassung:
This detailed volume provides a toolbox for designing constructs, tackling expression and solubility issues, handling membrane proteins and protein complexes, and exploring innovative engineering of E. coli. The topics are largely grouped under four parts: high-throughput cloning, expression screening, and optimization of expression conditions, protein production and solubility enhancement, case studies to produce challenging proteins and specific protein families, as well as applications of E. coli expression. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Heterologous Gene Expression in E. coli: Methods and Protocols serves molecular biologists, biochemists and structural biologists, those in the beginning of their research careers to those in their prime, to give both an historical and modern overview of the methods available to express their genes of interest in this exceptional organism.
Umfang:
1 Online-Ressource (XIV, 429 Seiten) 92 Illustrationen, 55 Illustrationen in Farbe
text file PDF
ISBN:
9781493968879
ISSN:
1940-6029 ;
DOI:
10.1007/978-1-4939-6887-9
Schlagworte:
Microbiology.
Bezugswerke:
Links: