Inclusion Bodies Methods and Protocols /

This detailed volume presents a series of protocols dealing with different aspects of inclusion body (IB) processing, from cloning procedures to purification of refolded product. Commencing with chapters on upstream processing, looking into different expression strategies for IB production, the book...

Ausführliche Beschreibung

Gespeichert in:
Hauptverfasser:
Kopp, Julian., Spadiut, Oliver.
Körperschaft:
SpringerLink (Online service)
Weitere Verfasser:
Kopp, Julian (HerausgeberIn, http://id.loc.gov/vocabulary/relators/edt), Spadiut, Oliver (HerausgeberIn, http://id.loc.gov/vocabulary/relators/edt)
Format:
Elektronisch E-Book
Sprache:
Englisch
Veröffentlicht:
New York, NY : Springer US : Imprint: Humana, 2023.
Ausgabe:
1st ed. 2023.
Zusammenfassung:
This detailed volume presents a series of protocols dealing with different aspects of inclusion body (IB) processing, from cloning procedures to purification of refolded product. Commencing with chapters on upstream processing, looking into different expression strategies for IB production, the book continues with downstream applications, highlighting early protein purification and subsequent analytics, as well as success stories of IB-based processes. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Inclusion Bodies: Methods and Protocols serves as an ideal resource for facilitating diverse aspects of IB processing.
Umfang:
XI, 272 p. 55 illus., 47 illus. in color. online resource.
text file PDF
ISBN:
9781071629307
ISSN:
1940-6029 ;
DOI:
10.1007/978-1-0716-2930-7
Schlagworte:
Biologicals.
Biopharmaceutics.
Proteins.
Biomaterials.
Pharmaceutical chemistry.
Biologics.
Biomaterials-Proteins.
Pharmaceutics.
Bezugswerke:
Links: